Tip in–light on: Advantages, challenges, and applications of combining AFM and Raman microscopy on biological samples
| Autor: | Prats‐Mateu, Batirtze, Gierlinger, Notburga |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
TERS
Microscopy Confocal confocal Raman microscopy Review Article scanning probe microscopies Microscopy Scanning Probe Microscopy Atomic Force Spectrum Analysis Raman Lignin biological materials nanomechanics Structure-Activity Relationship co‐located AFM Raman near‐field Raman Feasibility Studies Biological Assay AFM nanochemistry Cellulose |
| Zdroj: | Microscopy Research and Technique |
| ISSN: | 1097-0029 1059-910X |
| Popis: | Scanning probe microscopies and spectroscopies, especially AFM and Confocal Raman microscopy are powerful tools to characterize biological materials. They are both non‐destructive methods and reveal mechanical and chemical properties on the micro and nano‐scale. In the last years the interest for increasing the lateral resolution of optical and spectral images has driven the development of new technologies that overcome the diffraction limit of light. The combination of AFM and Raman reaches resolutions of about 50–150 nm in near‐field Raman and 1.7–50 nm in tip enhanced Raman spectroscopy (TERS) and both give a molecular information of the sample and the topography of the scanned surface. In this review, the mentioned approaches are introduced, the main advantages and problems for application on biological samples discussed and some examples for successful experiments given. Finally the potential of colocated AFM and Raman measurements is shown on a case study of cellulose‐lignin films: the topography structures revealed by AFM can be related to a certain chemistry by the colocated Raman scan and additionally the mechanical properties be revealed by using the digital pulsed force mode. Microsc. Res. Tech. 80:30–40, 2017. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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