| Popis: |
Lung cancer is one of the most common carcinomas worldwide. It is of value to know whether lung is more vulnerable to carcinogens than other tissues. In this study we compared the carcinogenic potential of 3,4-benzopyrene administered by intrapulmonary injection or subcutaneous injection. Ninety rats were randomly divided into three groups (n=30/group). Rats under deep anesthesia were treated with 3,4-benzopyrene by intrapulmonary injection or scapular subcutaneous injection, or with the vehicle by subcutaneous injection. The Rats were sacrificed when they developed advanced somatic sarcomas or severe dyspnea and the rats without severe phenotypes were sacrificed after 1 year. The tumors were isolated and examined with H&E staining. The expression of Bcl-2, CYP1A1, and NF-κB mRNA and protein in somatic sarcoma and lung carcinoma tissues was examined by in situ hybridization, immunohistochemistry, and Western blot. No tumor development was observed in the control rats. Fifteen of the 30 rats receiving an intrapulmonary injection of 3,4-benzopyrene developed lung carcinomas, whereas all 30 rats treated with subcutaneous injection developed a malignant neoplasm under the skin. Positive Bcl-2, CYP1A1, and NF-κB protein staining was observed in lung carcinoma and subcutaneous malignant neoplasm but Bcl-2 protein expression was much stronger in subcutaneous malignant neoplasms than in lung carcinoma. The expression pattern of Bcl-2, CYP1A1, and NF-κB mRNA in lung carcinoma and subcutaneous malignant neoplasms was consistent with its protein expression. Our results indicated that the lung is not more vulnerable to carcinogens than other tissues. The lung may acquire a protective mechanism against lung carcinogenesis through regulation of Bcl-2 expression. |
