Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures

Autor:	Huaqiong, Li, Yee Shan, Wong, Feng, Wen, Kee Woei, Ng, Gary Ka Lai, Ng, Subbu S, Venkatraman, Freddy Yin Chiang, Boey, Lay Poh, Tan
Rok vydání:	2012
Předmět:	Male Adolescent Tissue Engineering Tissue Scaffolds Cell Survival Lasers Polyesters Cell Differentiation Mesenchymal Stem Cells Electrochemical Techniques Extracellular Matrix Biomimetic Materials Humans Muscle Skeletal Biomarkers Cells Cultured Cell Proliferation
Zdroj:	Macromolecular bioscience. 13(3)
ISSN:	1616-5195
Popis:	Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e0db907f5a6d6d0d360bc9b2dcaedc37 https://pubmed.ncbi.nlm.nih.gov/23233197 Zobrazit plný text záznamu Plný text