Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection
| Autor: | Amit, Gautam, Richard J, Grainger, J, Vilardell, J David, Barrass, Jean D, Beggs |
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| Rok vydání: | 2015 |
| Předmět: |
Adenosine Triphosphatases
Saccharomyces cerevisiae Proteins Protein Conformation Ribonucleoprotein U4-U6 Small Nuclear RNA Splicing Genes Fungal Molecular Sequence Data RNA-Binding Proteins RNA Fungal Saccharomyces cerevisiae Alternative Splicing RNA Precursors Spliceosomes Humans Nucleic Acid Conformation RNA Amino Acid Sequence RNA Splice Sites RNA Splicing Factors Carrier Proteins Gene Deletion RNA Helicases Ribonucleoprotein U5 Small Nuclear |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Pre-mRNA splicing involves two transesterification steps catalyzed by the spliceosome. How RNA substrates are positioned in each step and the molecular rearrangements involved, remain obscure. Here, we show that mutations in PRP16, PRP8, SNU114 and the U5 snRNA that affect this process interact genetically with CWC21, that encodes the yeast orthologue of the human SR protein, SRm300/SRRM2. Our microarray analysis shows changes in 3′ splice site selection at elevated temperature in a subset of introns in cwc21Δ cells. Considering all the available data, we propose a role for Cwc21p positioning the 3′ splice site at the transition to the second step conformation of the spliceosome, mediated through its interactions with the U5 snRNP. This suggests a mechanism whereby SRm300/SRRM2, might influence splice site selection in human cells. |
| Databáze: | OpenAIRE |
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