| Autor: |
K, De Clercq, P, Mertens, I, De Leeuw, C, Oura, P, Houdart, A C, Potgieter, S, Maan, J, Hooyberghs, C, Batten, E, Vandemeulebroucke, I M, Wright, N, Maan, F, Riocreux, A, Sanders, Y, Vanderstede, K, Nomikou, M, Raemaekers, A, Bin-Tarif, A, Shaw, M, Henstock, E, Bréard, E, Dubois, C, Gastaldi-Thiéry, S, Zientara, B, Verheyden, F, Vandenbussche |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Transboundary and emerging diseases. 56(9-10) |
| ISSN: |
1865-1682 |
| Popis: |
An EDTA-blood sample from a cow without clinical signs, which gave early birth to a newborn calf that died soon after delivery, was shown to be positive for bluetongue virus (BTV)-RNA using a group-specific real-time RT-PCR (RT-qPCR). In-house serotype-specific RT-qPCR assays for bluetongue virus serotype 1 (BTV-1), -6 and -8 all gave negative results. Subsequent assays were carried out using conventional (gel-based) RT-PCR primers for all 25 BTV serotypes and only two primer sets, both specific for BTV-11, gave bands of the expected size. The cDNAs generated were sequenced and comparisons of the genome segment 2 sequence with that of the modified 'live' vaccine strain of BTV-11 from South Africa showed 100% identity. A survey of all ruminants in a 1-km area around the first positive farm using a BTV-11 serotype-specific RT-qPCR revealed five other holdings with in total nine BTV-11 positive animals. A cross-sectional monitoring of dairy cattle in Belgium showed an overall prevalence of 3.8% on herd level and 0.2% on animal level. A BTV-11 has been introduced into the Belgian cattle herd during the 2008 vector season. The source of the infection and the way by which the virus was introduced are unknown. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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