| Autor: |
Austin B, Keeler, Amy L, Van Deusen, Irene C, Gadani, Corey M, Williams, Sarah M, Goggin, Ashley K, Hirt, Shayla A, Vradenburgh, Kristen I, Fread, Emily A, Puleo, Lucy, Jin, O Yipkin, Calhan, Christopher D, Deppmann, Eli R, Zunder |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Nature neuroscience. 25(11) |
| ISSN: |
1546-1726 |
| Popis: |
Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single-cell mass cytometry on dorsal root ganglia from C57/BL6 mice of both sexes, with litter replicates collected daily from embryonic day 11.5 to postnatal day 4. Measuring nearly 3 million cells, we quantified 30 molecularly distinct somatosensory glial and 41 distinct neuronal states across all timepoints. Analysis of differentiation trajectories revealed rare cells that co-express two or more Trk receptors and over-express stem cell markers, suggesting that these neurotrophic factor receptors play a role in cell fate specification. Comparison to previous RNA-based studies identified substantial differences between many protein-mRNA pairs, demonstrating the importance of protein-level measurements to identify functional cell states. Overall, this study demonstrates that mass cytometry is a high-throughput, scalable platform to rapidly phenotype somatosensory tissues. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full text from SpringerLink