| Autor: |
Jesús Alfonso, Sánchez Viafara, Gisvani Lopes, de Vasconcelos, Renata, Maculan, Nadja Gomes, Alves, Marcos Brandao Dias, Ferreira, Mateus José, Sudano, Gisele Zoccal, Mingoti, Giovana Barros, Nunes, Renato Ribeiro, de Lima, Roberti Martins, Drumond, Raphael Nunes, Dos Santos, Marcos Nogueira, Eberlin, Fernanda, Negrão, Mariana Aragão M, Donato, Christina A, Peixoto, José, Camisão de Souza |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Reproduction, fertility, and development. 34(9) |
| ISSN: |
1031-3613 |
| Popis: |
The effect of L-165041 (PPARδ-agonist) on decreasing apoptosis and intracellular lipid content was assessed in fresh and vitrified-warmed in vitro -produced bovine embryos. It was hypothesised that the addition of L-165041 to the culture medium enhances development and cryopreservation. Oocytes were allocated to one of two treatments: control-standard culture medium, or L-165041 added to the medium on day1 with no media change. Ultrastructure, cleavage, and blastocyst rates were evaluated in fresh, and in post-vitrification cultured embryos by optical and electronic microscopy. A subset of fresh embryos were fixed for TUNEL assay and for Sudan-Black-B histochemical staining. Vitrified-warmed embryos were assessed using MALDI-MS technique. Cleavage and blastocyst rates (control 49.4±5.2, L-165041 51.8±4.3) were not influenced by L-165041. The proportion of inner cell mass cells (ICM) was higher in fresh embryos, and the rate of total and ICM apoptosis was lower in L-165041. In warmed-embryos, total and ICM apoptosis was lower in L-165041. The overall hatching rate was higher in L-165041 (66.62±2.83% vs 53.19±2.90%). There was less lipid accumulation in fresh L-165041-embryos. In conclusion, the use of L-165041 is recommended to improve the viability of in vitro -derived bovine embryos. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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