Gene Expression Analysis by Reverse Transcription Quantitative PCR

Autor:	Eva M, Campion, Sinéad T, Loughran
Rok vydání:	2021
Předmět:	RNA Bacterial Bacterial Proteins Helicobacter pylori Gene Expression Profiling Humans Gene Expression Regulation Bacterial Real-Time Polymerase Chain Reaction Helicobacter Infections
Zdroj:	Methods in molecular biology (Clifton, N.J.). 2283
ISSN:	1940-6029
Popis:	The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a rapid detection technology that allows the amplification and quantification of specific RNA transcripts. RT-qPCR has increasingly been adopted for the detection and quantification of H. pylori across a range of sample types and applications. In addition, it is widely used to monitor host gene expression in cells and tissues in response to H. pylori infection . Outlined here is a two-step protocol that can be employed to analyze gene expression in H. pylori or H. pylori-infected samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::df8654d6ec827463ee03c04a8ac48278 https://pubmed.ncbi.nlm.nih.gov/33765310 Zobrazit plný text záznamu