Perivenous expression of the mRNA of the three hypoxia-inducible factor alpha-subunits, HIF1alpha, HIF2alpha and HIF3alpha, in rat liver
| Autor: | T, Kietzmann, Y, Cornesse, K, Brechtel, S, Modaressi, K, Jungermann |
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| Rok vydání: | 2001 |
| Předmět: |
Antigens
Polyomavirus Transforming Molecular Sequence Data Transfection Basic Helix-Loop-Helix Transcription Factors Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Luciferases Erythropoietin Cells Cultured Cell Nucleus Sequence Homology Amino Acid Nuclear Proteins Hypoxia-Inducible Factor 1 alpha Subunit Rats DNA-Binding Proteins Repressor Proteins Liver Hepatocytes Trans-Activators Hypoxia-Inducible Factor 1 Apoptosis Regulatory Proteins Transcription Factors Research Article |
| Zdroj: | The Biochemical journal. 354(Pt 3) |
| ISSN: | 0264-6021 |
| Popis: | The cDNAs of three hypoxia-inducible factor (HIF) alpha-subunits were cloned from RNA of primary rat hepatocytes by reverse transcriptase PCR. All three cDNAs encoded functionally active proteins, of 825, 874 and 662 amino acids. After transfection they were able to activate luciferase activity of a luciferase gene construct containing three HIF-responsive elements. The mRNAs of the rat HIF alpha-subunits were expressed predominantly in the perivenous zone of rat liver tissue; the nuclear HIFalpha proteins, however, did not appear to be zonated. |
| Databáze: | OpenAIRE |
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