| Autor: |
Guangjing, Zou, Jingzhi, Su, Yujie, Zhang, Fang, Li, Changqi, Li, Xinling, Meng |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. 45(8) |
| ISSN: |
1672-7347 |
| Popis: |
To investigate the effects of environmental enrichment on cognitive behavior and the expression of adenosine triphosphate binding cassette transporter A7 (ABCA7) in hippocampus of the adolescent mice with high fat diet.A total of healthy 3-week-old male C57BL/6J mice were randomly divided into 3 groups: a control (Con) group, a high fat diet (HFD) group, and a high fat diet+environmental enrichment (HFD+EE) group, with 10 mice in each group. The Con group was given normal diet. The HFD group was given high fat diet. The HFD+EE group was given high fat diet; at the same time, they treated by environmental enrichment. After 10 weeks, open field test was used to detect activity. Novel object recognition test and Y maze test were used to detect cognitive behavior. After the test, the brain was collected and used to detect the protein expression of ABCA7 in the hippocampus by immunohistochemistry and Western blotting. And quantitative RT-PCR (RT-qPCR) was used to detect the ABCA7 mRNA expression level in the hippocampus.There was no significant difference in the total movement distance in the mice among the 3 groups (High fat diet in adolescent can impair cognitive function with a decrease in the expression of ABCA7 in hippocampus, which can be ameliorate by environmental enrichment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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