| Autor: |
Chao, Yang, Lei, Ji, Wen, Yue, Ruo-Yong, Wang, Yan-Hua, Li, Jia-Fei, Xi, Xiao-Yan, Xie, Li-Juan, He, Xue, Nan, Xue-Tao, Pei |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 18(4) |
| ISSN: |
1009-2137 |
| Popis: |
The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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