| Autor: |
M M, Montrel', B I, Sukhorukov |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia biologiia. 23(3) |
| ISSN: |
0026-8984 |
| Popis: |
The effects of hydrogen ions binding to DNA on its secondary structure and B to A transition were studied by methods of X-ray diffraction and infrared spectroscopy. Helical parameters of DNA molecules with different degrees of protonation were determined. It was shown that H+-ions binding stabilize the B form of DNA in fibers in the wide range of water and inorganic salt content. Only 0.03 H+-ions bound to each nucleotide are sufficient to prevent B to A transition caused by a relative humidity decrease in DNA fibers, containing 4% of NaCl. The effective stabilization of the DNA B form by H+-ions binding is explained by modifications in DNA - solvent molecules interactions, especially in the major groove of double helices. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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