The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity
| Autor: | John, Butler, Neil, Smyth, Robert, Broadbridge, Claire E, Council, Anthony G, Lee, Claire J, Stocker, David C, Hislop, Jonathan R S, Arch, Michael A, Cawthorne, J, Malcolm East |
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| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
Male animal structures Sarcolipin Proteolipids Recombinant Fusion Proteins Muscle Proteins Mice Transgenic Diet High-Fat Article Sarcoplasmic Reticulum Calcium-Transporting ATPases Mice SERCA Animals Obesity Muscle Skeletal musculoskeletal neural and ocular physiology High fat diet Body Weight Thermogenesis Up-Regulation cardiovascular system Energy expenditure Energy Metabolism tissues Oligopeptides |
| Zdroj: | Archives of Biochemistry and Biophysics |
| ISSN: | 1096-0384 |
| Popis: | Highlights • Sarcolipin is insufficient to affect thermogenic activity of SERCA in mouse muscle. • The ratio of SLN to SERCA in total limb skeletal muscle is Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet. |
| Databáze: | OpenAIRE |
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