Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia
| Autor: | Vivian, Luchsinger, Yara, Prades, Mauricio, Ruiz, Rolando, Pizarro, Patricio, Rossi, Luis, Lizama, María Luisa, Garmendia, Angela, Meza, Carmen, Larrañaga, Luis F, Avendaño |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Aged 80 and over Male diagnosis Luminex xTAG RVP polymerase chain reaction Pneumonia Viral community‐acquired pneumonia Middle Aged Real-Time Polymerase Chain Reaction Sensitivity and Specificity Community-Acquired Infections Hospitalization Young Adult Molecular Diagnostic Techniques Nasopharynx respiratory viruses Viruses Humans Female Reagent Kits Diagnostic Fluorescent Antibody Technique Indirect Research Articles Aged Research Article |
| Zdroj: | Journal of Medical Virology |
| ISSN: | 1096-9071 |
| Popis: | Community‐acquired pneumonia (CAP) is the third cause of death worldwide. Viruses are frequently detected in adult CAP. Highly sensitive diagnostic techniques should be used due to poor viral shedding. Different sampling methods can affect viral detection, being necessary to establish the optimal type of sample for identifying respiratory viruses in adults. The detection rates of respiratory viruses by Luminex xTAG® RVP fast assay, real time RT‐PCR (rtRT‐PCR) (Sacace®), and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 47.5% for Luminex®, 42.5% for rtRT‐PCR (P = 0.3), and 2.7% for IFA (2.7%) (P |
| Databáze: | OpenAIRE |
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