Colony-Forming Ability and Residual Foci of DNA Repair Proteins in Human Lung Fibroblasts Irradiated with Subpicosecond Beams of Accelerated Electrons

Autor: N S, Babayan, D V, Guryev, N Yu, Vorobyeva, B A, Grigoryan, G L, Tadevosyan, L S, Apresyan, A K, Chigasova, E I, Yashkina, S M, Rodneva, A A, Tsishnatti, Yu A, Fedotov, N K, Sarkisyan, A T, Manukyan, R M, Aroutiounian, A N, Osipov
Rok vydání: 2021
Předmět:
Zdroj: Bulletin of experimental biology and medicine. 172(1)
ISSN: 1573-8221
Popis: We performed a comparative study of the colony-forming ability and the number of residual foci of DNA repair proteins in cultured human lung fibroblasts (MRC-5 cell line) after exposure to subpicosecond beams of accelerated electrons with an energy of 3.6 MeV and quasi-continuous radiation (accelerated electrons with an energy of 4 MeV and X-rays). The yield of damages causing reproductive cell death after pulsed subpicosecond radiation exposure was higher by ~1.8 times than after quasi-continuous radiation exposure. The quantitative yield of residual γH2AX foci (phosphorylated H2AX histone, a protein marker of DNA double breaks) in cells irradiated with subpicosecond beams of accelerated electrons was shown to be ~2.0- 2.5-fold higher than in cells irradiated with quasi-continuous beams of accelerated electrons.
Databáze: OpenAIRE
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