| Autor: |
L-G, Dong, F-F, Lu, J, Zu, W, Zhang, C-Y, Xu, G-L, Jin, X-X, Yang, Q-H, Xiao, C-C, Cui, R, Xu, S, Zhou, J-N, Zhu, T, Shen, G-Y, Cui |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
European review for medical and pharmacological sciences. 24(21) |
| ISSN: |
2284-0729 |
| Popis: |
The aim of this study was to explore the effect of micro ribonucleic acid (miR)-133b on 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis in the Parkinson's disease (PD) model.PC12 cells were induced by different concentrations of MPP+ to establish the PD cell model. Subsequently, the survival rate of PC12 cells was detected using Cell Counting Kit-8 (CCK-8) assay. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of miR-133b in the PD model induced by different concentrations of MPP+. Next, PC12 cells were transfected with miR-133b mimic and miR-negative control (NC), and divided into MPP+ group, MPP+ + miR-NC group and MPP+ + miR-133b mimic group. Transfection efficiency was verified using qRT-PCR. The apoptosis of cells was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Moreover, the expressions of extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated (p)-ERK1/2 were determined using Western blotting.After MPP+ treatment, the survival rate of PC12 cells significantly declined (p0.05). MPP+ exhibited toxicity against PC12 cells in a concentration-dependent manner. Meanwhile, cell survival rate decreased remarkably with the increase of MPP+ concentration (p0.05). With increased concentration of MPP+, the expression of miR-133b in the PD cell model declined significantly (p0.05). The apoptosis of PC12 cells was remarkably inhibited by overexpression of miR-133b in the PD cell model (p0.05). In addition, the protein expression of p-ERK1/2 in PC12 cells was notably reduced after overexpression of miR-133b in the PD cell model (p0.05).MiR-133b is lowly expressed in the PD cell model. Furthermore, overexpression of miR-133b inhibits cell apoptosis in the PD cell model by regulating the ERK1/2 signaling pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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