[Directed reconstruction of the influenza virus hemagglutinin gene]

Autor:	V A, Petrenko, S M, Kipriianov, L N, Semenova, A N, Boldyrev, P I, Pozdniakov, A M, Eroshkin, G F, Sivolobova
Rok vydání:	1990
Předmět:	Base Sequence Chimera Genes Bacterial Molecular Sequence Data Restriction Mapping DNA Recombinant Escherichia coli Mutagenesis Site-Directed Hemagglutinins Viral Electrophoresis Polyacrylamide Gel Gene Expression Regulation Bacterial Cloning Molecular Orthomyxoviridae
Zdroj:	Molekuliarnaia biologiia. 24(5)
ISSN:	0026-8984
Popis:	A new approach to create chimeric genes by directed exchange of oligonucleotide fragments was developed. By oligonucleotide-directed mutagenesis a few deletion mutants of the influenza virus hemagglutinin (HA) gene were obtained. These variants of HA gene contain unique restriction sites in DNA regions coding for the A and B epitopes of the HA molecule. The obtained special vectors may be used for cloning DNA fragments coding for new amino acid sequences in internal sites of the HA gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::dd178718dcc02f0b54ec571b3438d162 https://pubmed.ncbi.nlm.nih.gov/2290420 Zobrazit plný text záznamu