| Autor: |
S V, Kornilova, P, Iasem, D N, Grigor'ev, L E, Kapinos, Ia, Totova, Iu P, Blagoĭ |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Biofizika. 42(3) |
| ISSN: |
0006-3029 |
| Popis: |
Using the method of differential scanning calorimetry, the DNA helix-coil transition studied in solutions (10(-3) M Na+, 10(-3) M tris HCl, pH 7.0) containing divalent metal ions (Mn2+ and Ca2+) at relative metal ion concentrations (Me/PDNA) ranging from 0.2 to 20. Dependences of the melting temperature and enthalpy on the ion relative concentration were stated. The fine structure of melting curves in DNA+Me complexes was observed. The resolution degree of this structure was shown to increase in the range of moderate ion concentrations. The data obtained were compared with values determined by UV-spectroscopy. Association constants were defined for Mn2+ and Ca2+ binding to DNA by the ligand theory. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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