| Autor: |
J M, Ramalho-Ortigão, S, Kamhawi, M B, Joshi, D, Reynoso, P G, Lawyer, D M, Dwyer, D L, Sacks, J G, Valenzuela |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
Insect molecular biology. 14(6) |
| ISSN: |
0962-1075 |
| Popis: |
We characterized a cDNA from Phlebotomus papatasi, PpChit1, which encodes a midgut specific chitinase and show the presence of a functional, blood-induced chitinolytic system in sand flies. PpChit1 is detected only in the midgut and is regulated by blood feeding. A recombinant protein (rPpChit1) produced in HEK 293-F cells exhibited a similar activity profile to that found in the native protein against several specific substrates, including an oligomeric glycol chitin and synthetic 4-methyl-umbelliferone labelled substrates. Western blotting showed that the native protein is recognized by mouse polyclonal antibodies against rPpChit1. Additionally, the rPpChit1 and the native chitinase displayed similar retention times in a HPLC size fractionation column. When added to rPpChit1 or to midgut lysates, PpChit1 sera reduced chitinolytic activity by 65-70%. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
