The transcription factor
| Autor: | Olga, Medina-Martinez, Meade, Haller, Jill A, Rosenfeld, Marisol A, O'Neill, Dolores J, Lamb, Milan, Jamrich |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male genetic structures Adolescent DNA Copy Number Variations Gene Dosage Wnt pathway Apoptosis Eye Polymorphism Single Nucleotide Young Adult Morphogenesis Sfrp2 Animals Humans Genetic Predisposition to Disease Eye Abnormalities Wnt Signaling Pathway CNV 16p11.2 Cell Proliferation Mice Knockout Gene Expression Regulation Developmental Infant eye diseases DNA-Binding Proteins Mice Inbred C57BL Phenotype Child Preschool Female Maz Transcription Factors Research Article |
| Zdroj: | Disease Models & Mechanisms article-version (VoR) Version of Record |
| ISSN: | 1754-8411 |
| Popis: | Wnt/β-catenin signaling has an essential role in eye development. Faulty regulation of this pathway results in ocular malformations, owing to defects in cell-fate determination and differentiation. Herein, we show that disruption of Maz, the gene encoding Myc-associated zinc-finger transcription factor, produces developmental eye defects in mice and humans. Expression of key genes involved in the Wnt cascade, Sfrp2, Wnt2b and Fzd4, was significantly increased in mice with targeted inactivation of Maz, resulting in abnormal peripheral eye formation with reduced proliferation of the progenitor cells in the region. Paradoxically, the Wnt reporter TCF-Lef1 displayed a significant downregulation in Maz-deficient eyes. Molecular analysis indicates that Maz is necessary for the activation of the Wnt/β-catenin pathway and participates in the network controlling ciliary margin patterning. Copy-number variations and single-nucleotide variants of MAZ were identified in humans that result in abnormal ocular development. The data support MAZ as a key contributor to the eye comorbidities associated with chromosome 16p11.2 copy-number variants and as a transcriptional regulator of ocular development. Summary: Our study has uncovered Maz as an important regulator of eye development in humans and mice, striving to elucidate the role of this gene in eye abnormalities associated with the human ch16p11.2 microdeletions and microduplications. |
| Databáze: | OpenAIRE |
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