| Autor: |
X M, Zhang, S, Huck, P, Dariavach, R, Spilliaert, A, Arnason, Z, Soua, G, Lefranc, M P, Lefranc |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Experimental and clinical immunogenetics. 12(4) |
| ISSN: |
0254-9670 |
| Popis: |
The polymerase chain reaction using oligonucleotides based on consensus sequences from other species allowed us to amplify minke whale (Balaenoptera acutorostrata) T cell receptor (TcR) gamma and delta constant genes. Two types of Cgamma clones were obtained which only differ by one mismatch. These minke whale Cgamma clones showed 83% nucleotide and 70% amino acid sequence similarity to the corresponding region of the human TRGC genes. The minke whale Cdelta sequences were all identical, and showed 86% nucleotide and 77.8% amino acid sequence similarity to the human TRDC gene. The whale Cgamma and Cdelta clones were used as probes for Southern blot analysis. The data confirmed that there is a unique Cdelta gene in minke whale. Two different Cgamma genes were detected, one of them also cross-hybridizing with a human Cgamma probe, suggesting that the Cgamma locus in minke whale consists of at least two different constant genes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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