| Autor: |
Lian-qun, Jia, Jun-yi, Feng, Guan-lin, Yang, Wen-na, Chen, Yang, Chen |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 27(7) |
| ISSN: |
1007-8738 |
| Popis: |
To observe the influence of tanshinone II A on the expression of toll like receptor 4(TLR4) and tumor necrosis factor-α(TNF-α) in cultured human umbilical veins endothelial cells EA.hy926 induced by LPS, and to investigate the molecular mechanism of tanshinone II A against atherosclerosis(AS).The EA.hy926 cells were cultured in vitro. The mRNA and protein expression of TLR4 were tested by RT-PCR and immunocytochemistry technique respectively. The mRNA and protein expression of TNF-α were detected by RT-PCR and ELISA technique respectively.The expression of TLR4 and TNF-α were both increased compared to normal group after stimulation of LPS(P0.05). The expression of TLR4 and TNF-α in tanshinone II A group were obviously inhibited compared to stimulation group(P0.05).The molecular mechanism of tanshinone II A against AS is probably to inhibit the expression of TLR4 and TNF-α. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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