Tissue-print hybridization using a non-radioactive probe for the detection of infectious bursal disease virus

Autor:	T L, Hathcock, J J, Giambrone
Rok vydání:	1992
Předmět:	Bursa of Fabricius Base Sequence Molecular Sequence Data Animals Nucleic Acid Hybridization RNA Viral Oligonucleotide Probes Chickens Infectious bursal disease virus Poultry Diseases Reoviridae Infections Specific Pathogen-Free Organisms
Zdroj:	Avian diseases. 36(2)
ISSN:	0005-2086
Popis:	Tissue-print hybridization was evaluated as a simplified means for detection of infectious bursal disease virus (IBDV) in the bursa of Fabricius from infected chickens. The assay employed a biotin-labeled synthetic oligonucleotide as a probe. The bound probe was detected using a color assay consisting of streptavidin conjugated to alkaline phosphatase. Bursae were imprinted onto nitrocellulose and then hybridized with the biotinylated probe. Bursal prints from IBDV-infected chickens were readily distinguished from control prints by color development and differences in signal intensity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::db71da4a02cd984c9aee48073814e245 https://pubmed.ncbi.nlm.nih.gov/1320859 Zobrazit plný text záznamu