Molecular cloning and expression of human alveolar macrophage cathepsin S, an elastinolytic cysteine protease
| Autor: | G P, Shi, J S, Munger, J P, Meara, D H, Rich, H A, Chapman |
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| Rok vydání: | 1992 |
| Předmět: |
Binding Sites
Base Sequence Pancreatic Elastase Molecular Sequence Data Gene Expression DNA Cysteine Proteinase Inhibitors Hydrogen-Ion Concentration Blotting Northern Cathepsins Polymerase Chain Reaction Recombinant Proteins Cell Line Elastin Cysteine Endopeptidases Macrophages Alveolar Autoradiography Humans RNA Electrophoresis Polyacrylamide Gel Amino Acid Sequence Cloning Molecular |
| Zdroj: | The Journal of biological chemistry. 267(11) |
| ISSN: | 0021-9258 |
| Popis: | Human alveolar macrophages (HAM) express an elastase activity of acidic pH optimum inhibitable by cysteine protease inhibitors. Recent studies indicate that the only known eukaryotic elastinolytic cysteine protease, cathepsin L, cannot completely account for this activity. In order to search for additional cysteine proteases with elastinolytic activity, low degeneracy oligonucleotide primers based on regions of strong homology among the known cysteine proteases were used to screen reverse-transcribed HAM RNA for cysteine proteases by the polymerase chain reaction. Among the cDNA sequences generated was a 493-base pair product highly homologous to bovine cathepsin S. Screening of a HAM cDNA eukaryotic expression library with this cDNA yielded a 1.7-kilobase full-length cDNA highly homologous to bovine cathepsin S (approximately 85% identical). This cDNA predicts a 331-amino acid preprocathepsin. Expression of this cDNA in COS cells revealed the active enzyme to be a single chain 28-kDa protease, as judged by active site labeling with a novel iodinated analogue of N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucylamido-(4-gua nido)butane (E-64). The recombinant enzyme was found to be elastinolytic toward 3H-labeled elastin (bovine ligamentum nuchae) at pH 5.5 but with 25% of this activity retained at pH 7.0. Labeling of HAM with the active site probe revealed these cells express a 28-kDa cysteine protease, and Northern blot analysis revealed the presence of a approximately 1.7-kilobase cathepsin S mRNA. These data establish that human macrophages express at least two cysteine proteases with elastinolytic activity. The relatively broad pH range of human cathepsin S activity suggests this enzyme may contribute to the contact-dependent elastase activity of live human alveolar macrophages. |
| Databáze: | OpenAIRE |
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