| Autor: |
E S, Ostanina, V P, Varlamov, G I, Iakovlev |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 44(1) |
| ISSN: |
0555-1099 |
| Popis: |
Inhibition of enzymatic activity of lipase (EC 3.1.1.3) from the fungus Candida rugosa and wheat (Triticum aestivum L.) germ by low-molecular-weight chitosan with an average molecular weight of 5.7 kDa in reactions of p-nitrophenyl palmitate cleavage was studied. Preincubation of lipases with chitosan, prior to addition of the substrate to solution, showed that equilibrium during the lipase-inhibitor complex formation was reached within 30 min. The inhibition constants for C. rugosa lipase and wheat germ lipase were 1.4 and 0.9 mM, respectively. The contribution of electrostatic interactions to the complex formation between chitosan and lipases is insignificant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
