Overexpression of Cks1 is associated with poor survival by inhibiting apoptosis in breast cancer
| Autor: | Xiao-Chun, Wang, Li-Li, Tian, Jing, Tian, Hai-Liang, Wu, Ai-Min, Meng |
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| Rok vydání: | 2008 |
| Předmět: |
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3 Blotting Western Gene Amplification MAP Kinase Kinase 1 Apoptosis Breast Neoplasms Middle Aged Flow Cytometry Prognosis Cyclin-Dependent Kinases Gene Expression Regulation Neoplastic Immunoenzyme Techniques Survival Rate Cell Movement Lymphatic Metastasis CDC2-CDC28 Kinases Humans Female Neoplasm Invasiveness RNA Small Interfering Carrier Proteins In Situ Hybridization Fluorescence Cell Proliferation Neoplasm Staging |
| Zdroj: | Journal of cancer research and clinical oncology. 135(10) |
| ISSN: | 1432-1335 |
| Popis: | The present study was undertaken to examine the amplification and expression status of Cks1 in breast cancer and its significance.The amplification and expression status of Cks1 gene was examined by FISH, real-time PCR and immunohistochemistry, respectively. RNA interference was used to detect the effects of Cks1 on migration, invasion, cell cycle progress and apoptosis of breast cancer cells.Cks1 gene amplification was highly correlated with protein overexpression. Overexpression of Cks1 was strongly associated with lymph node metastasis and poor prognosis (P = 0.000, 95% CI (0.00-0.02); P = 0.008, 95% CI (0.001-0.05), respectively). Knockdown of Cks1 expression by RNA interference inhibited the cell cycle progress, migration and invasion ability of breast cancer cells. Moreover, overexpression of Cks1 inhibited apoptosis of breast cancer cells through MEK-Erk pathway.Cks1 may be considered as potential novel prognostic markers and targets for the future development of specific therapeutic interventions in breast cancer. |
| Databáze: | OpenAIRE |
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