The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii x Aloe aristata (Aloaceae), to its progeny

Autor:	C, Takahashi, I J, Leitch, A, Ryan, M D, Bennett, P E, Brandham
Rok vydání:	1997
Předmět:	Recombination Genetic Meiosis Plants Medicinal Gene Frequency Staining and Labeling Chimera Oxazines Aloe Plants Chromosomes Crosses Genetic In Situ Hybridization Metaphase
Zdroj:	Chromosoma. 105(6)
ISSN:	0009-5915
Popis:	Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::da2801233f7f3d7668039965f5022b2e https://pubmed.ncbi.nlm.nih.gov/9087376 Zobrazit plný text záznamu Full text from SpringerLink