Spi-1 oncogene activation in Rauscher and Friend murine virus-induced acute erythroleukemias
| Autor: | F, Moreau-Gachelin, D, Ray, N J, de Both, M J, van der Feltz, P, Tambourin, A, Tavitian |
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| Rok vydání: | 1990 |
| Předmět: |
Gene Rearrangement
Transcriptional Activation Leukemia Experimental Genes Viral Transcription Genetic Oncogenes Rauscher Virus Friend murine leukemia virus Gene Expression Regulation Neoplastic Leukemia Virus Murine Mice Mice Inbred DBA Acute Disease Tumor Cells Cultured Animals Leukemia Erythroblastic Acute Spleen Focus-Forming Viruses |
| Zdroj: | Leukemia. 4(1) |
| ISSN: | 0887-6924 |
| Popis: | The Friend viruses, like the Rauscher virus, cause murine acute erythroleukemias which evolve in a similar multistep process. In previous studies it has been described that the late malignant proerythroblastic transformation induced by the polycythemia-inducing strain of Friend spleen focus-forming virus (SFFVP) is correlated with Spi-1 oncogene activation by insertional mutagenesis. In this paper we report that Spi-1 genomic rearrangements were also observed in 90% of tumors induced by the anemia-inducing strain of Friend spleen focus-forming virus (SFFVA) and in all Rauscher-induced tumors analyzed. SFFVA and Rauscher proviral insertions occurred in the viral integration cluster previously characterized in SFFVP-induced tumors. The Spi-1 1.4-Kb messenger RNA was found highly expressed in all SFFVA and Rauscher-induced malignant cells as compared to normal tissues. The nucleotide sequence of Spi-1 cDNA isolated from a library constructed from SFFVA-induced tumor cells revealed no difference between the Spi-1 gene transcripts expressed in both SFFVP and SFFVA-induced leukemic cells. These results indicate that Spi-1 gene activation is a general feature in the malignant proerythroblastic transformation which occurs in mice infected with Friend and Rauscher viruses. |
| Databáze: | OpenAIRE |
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