| Autor: |
S B, Duan, S S, Wei, H M, Wang, S H, Ding, Y Z, Chen, J J, Tian, Y J, Wang, W, Chen, J, Chen, Q L, Meng |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia biologiia. 55(6) |
| ISSN: |
0026-8984 |
| Popis: |
When expressing streptavidin recombinant polypeptide on magnetosomes (called bacterial magnetic nanoparticles, or BMPs), the presence of endogenous bacterial biotin might be detrimental. In the study, the streptavidin monomer fragment (S1-116) was fused with the intein N-terminal (termed precursor S1-116-IN), and S1-116-IN was expressed in E. coli (BL21). Meanwhile, the SA117-160 fragment was fused with the C-terminal intein, and then this chimeric polypeptide was expressed on magnetosomes by fusion with magnetosome membrance protein MamF. In the in vitro protein splicing system, the purified engineered magnetosomes (BMP-SA117-160-IC) and the S1-116-IN precursor were mixed. Intein-mediated trans-splicing reaction was induced to produce the functional magnetic beads BMP-SA. Our results indicate that intein-mediated protein trans-splicing may lead to efficient synthesis of the recombinant streptavidin on the magnetosomes, showing its promising potential to produce other functional magnetic nanoparticles. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
