Partial sequencing and tissue distribution of the canine isoforms of steroid 5alpha-reductase type I and type II
| Autor: | P N, Span, A, van Bokhoven, A G, Smals, C G, Sweep, J A, Schalken |
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| Rok vydání: | 2000 |
| Předmět: |
Electrophoresis
Agar Gel Male Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Molecular Sequence Data Prostate DNA Sequence Analysis DNA Blotting Northern Isoenzymes Dogs 3-Oxo-5-alpha-Steroid 4-Dehydrogenase Animals RNA Scintillation Counting Amino Acid Sequence Cloning Molecular Sequence Alignment Chromatography High Pressure Liquid DNA Primers |
| Zdroj: | The Prostate. 44(3) |
| ISSN: | 0270-4137 |
| Popis: | The dog is regarded to be a valid model to test the effects of 5alpha-reductase inhibitors on prostatic growth. However, limited information is available on the characteristics or even existence of 5alpha-reductase isozymes in this species.Here, we set out to clone the cDNA of the dog isoforms of 5alpha-reductase type I and type II by a degenerate cloning strategy and to assess the tissue distribution of both transcripts and the enzymatic activity of the isozymes.We identified two clones with homology to the human 5alpha-reductase isoforms type I and type II to be expressed in dog prostate. At the amino-acid level, these partial clones were found to exhibit a homology with their human counterparts of 83% and 88%, respectively. The expression levels of 5alpha-reductase mRNA were screened by RT-PCR in a number of dog tissues. No correlation was found between tissue mRNA expression and enzymatic 5alpha-reductase activities.The present study describes the partial cloning of the dog 5alpha-reductase isozymes and their tissue distribution. These results provide additional data for the use of the dog as an animal model to investigate the role of 5alpha-reductase isozymes in steroid metabolism. |
| Databáze: | OpenAIRE |
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