Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology

Autor:	Elina B, Reinoso, Susana G, Bettera
Rok vydání:	2015
Předmět:	DNA Bacterial Molecular Epidemiology Humans Curriculum Educational Measurement Problem-Based Learning Saccharomyces cerevisiae Students Polymerase Chain Reaction Disease Outbreaks Random Amplified Polymorphic DNA Technique
Zdroj:	Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology. 44(4)
ISSN:	1539-3429
Popis:	In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(4):391-396, 2016.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d9885b943da1b7ca9b15f9279793e5ad https://pubmed.ncbi.nlm.nih.gov/26898662 Zobrazit plný text záznamu Plný text