Sex hormones regulate the contribution of PKCepsilon and PKA signalling in inflammatory pain in the rat
| Autor: | O A, Dina, K O, Aley, W, Isenberg, R O, Messing, J D, Levine |
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| Rok vydání: | 2001 |
| Předmět: |
Central Nervous System
Male MAP Kinase Kinase 1 Pain Protein Kinase C-epsilon Protein Serine-Threonine Kinases Dinoprostone Rats Sprague-Dawley Mice Animals Enzyme Inhibitors Gonadal Steroid Hormones Protein Kinase C Inflammation Mice Knockout Mitogen-Activated Protein Kinase Kinases Neurons Sex Characteristics omega-N-Methylarginine Estradiol Adrenergic Agonists Cyclic AMP-Dependent Protein Kinases Rats Isoenzymes Intercellular Signaling Peptides and Proteins Female Nitric Oxide Synthase Peptides Signal Transduction |
| Zdroj: | The European journal of neuroscience. 13(12) |
| ISSN: | 0953-816X |
| Popis: | We have evaluated the contribution of differences in second messenger signalling to sex differences in inflammatory pain and its control by sex hormones. In normal male but not female rats, epinephrine-induced mechanical hyperalgesia was antagonized by inhibitors of protein kinase Cepsilon (PKCepsilon), protein kinase A (PKA) and nitric oxide synthetase (NOS). Similarly, in PKCepsilon knockout mice, a contribution of PKCepsilon to epinephrine-dependent mechanical hyperalgesia occurred in males only. In contrast, hyperalgesia induced by prostaglandin E2, in both females and males, was dependent on PKA and NO. In both sexes, inhibitors of mitogen-activated protein kinase/extracellular-signal related kinase kinase (MEK) inhibited epinephrine hyperalgesia. In gonadectomized females, the second messenger contributions to epinephrine hyperalgesia demonstrated the pattern seen in males. Administration of oestrogen to gonadectomized females fully reconstituted the phenotype of the normal female. These data demonstrate gender differences in PKCepsilon, PKA and NO signalling in epinephrine-induced hyperalgesia which are oestrogen dependent and appear to be exerted at the level of the beta-adrenergic receptor or the G-protein to which it is coupled. |
| Databáze: | OpenAIRE |
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