Borealin–nucleosome interaction secures chromosome association of the chromosomal passenger complex
| Autor: | Abad, Maria A., Ruppert, Jan G., Buzuk, Lana, Wear, Martin, Zou, Juan, Webb, Kim M., Kelly, David A., Voigt, Philipp, Rappsilber, Juri, Earnshaw, William C., Jeyaprakash, A. Arockia |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Protein Folding
Survivin Centromere Mitosis Cell Cycle Proteins macromolecular substances Protein Serine-Threonine Kinases Chromosomes Mass Spectrometry Histones Xenopus laevis Report Chromosome Segregation Animals Aurora Kinase B Humans Phosphorylation Research Articles technology industry and agriculture Intracellular Signaling Peptides and Proteins Recombinant Proteins Nucleosomes Kinetics Microscopy Fluorescence Cell Division HeLa Cells Protein Binding |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Abad et al. report that the chromosomal passenger complex (CPC), an essential regulator of error-free chromosome segregation, is targeted to chromosomes via Borealin-mediated direct interaction with nucleosomes. This is critical for subsequent histone modification–dependent centromere enrichment and function of the CPC during metaphase. Chromosome association of the chromosomal passenger complex (CPC; consisting of Borealin, Survivin, INCENP, and the Aurora B kinase) is essential to achieve error-free chromosome segregation during cell division. Hence, understanding the mechanisms driving the chromosome association of the CPC is of paramount importance. Here using a multifaceted approach, we show that the CPC binds nucleosomes through a multivalent interaction predominantly involving Borealin. Strikingly, Survivin, previously suggested to target the CPC to centromeres, failed to bind nucleosomes on its own and requires Borealin and INCENP for its binding. Disrupting Borealin–nucleosome interactions excluded the CPC from chromosomes and caused chromosome congression defects. We also show that Borealin-mediated chromosome association of the CPC is critical for Haspin- and Bub1-mediated centromere enrichment of the CPC and works upstream of the latter. Our work thus establishes Borealin as a master regulator determining the chromosome association and function of the CPC. |
| Databáze: | OpenAIRE |
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