Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR
| Autor: | R G, Jain, L G, Andrews, K M, McGowan, F, Gao, J D, Keene, P P, Pekala |
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| Rok vydání: | 1995 |
| Předmět: |
Base Composition
Glucose Transporter Type 1 Base Sequence Monosaccharide Transport Proteins Molecular Sequence Data ELAV-Like Protein 2 RNA-Binding Proteins Nerve Tissue Proteins 3T3 Cells Ligands Mice Drosophila melanogaster ELAV Proteins Sequence Homology Nucleic Acid Animals Humans Nucleic Acid Conformation RNA Messenger |
| Zdroj: | Nucleic acids symposium series. (33) |
| ISSN: | 0261-3166 |
| Popis: | Hel-N1, is an RRM protein which is a mammalian homologue of the Drosophila melanogaster RNA binding protein, ELAV (embryonic lethal abnormal vision). Hel-N1 binds to RNA containing short stretches of uridylates similar to those found in the 3' untranslated regions (3'-UTRs) of oncoprotein and cytokine mRNAs. The GLUT1 glucose transporter has an extensive 3' UTR that is AU-rich reminiscent of the 3'UTR of an oncogene mRNA. An in vitro RNA binding assay using Hel-N1 demonstrated binding to a specific portion of the GLUT1 3'UTR. Analysis of the folding pattern of this region depicted the retention of a stem loop structure, wherein the loop is composed of a stretch of uridylates. To further analyze the potential function of Hel-N1, stable transfectants were made in the 3T3-L1 cell line. The transfectants have been characterized, and the presence of the Hel-N1 DNA and protein verified. Data indicate Hel-N1 is a ligand for GLUT1 and its binding affects the stability and translatability of the GLUT1 message. |
| Databáze: | OpenAIRE |
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