In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina

Autor:	Devi Krishna Priya, Karunakaran, Rahul, Kanadia
Rok vydání:	2017
Předmět:	Mice Electroporation Gene Expression Regulation Microscopy Fluorescence Genes Reporter Gene Transfer Techniques Animals Gene Expression Retina Morpholinos
Zdroj:	Methods in molecular biology (Clifton, N.J.). 1565
ISSN:	1940-6029
Popis:	Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d8386cfc000301764c48dd185a72b995 https://pubmed.ncbi.nlm.nih.gov/28364246 Zobrazit plný text záznamu