| Autor: |
G E, Webley, P L, Marsden, P G, Knight |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Biology of reproduction. 50(6) |
| ISSN: |
0006-3363 |
| Popis: |
There is an increase in plasma concentrations of immunoreactive (ir) inhibin unaccompanied by a rise in plasma progesterone during early pregnancy in the marmoset monkey. We investigated the potential involvement of hCG and prostaglandin E2 (PGE2) in stimulating a selective increase in inhibin concentrations by measuring the production of ir-alpha-inhibin and progesterone by dispersed luteal cells cultured under serum-free conditions. After one day, hCG had no effect on progesterone production by the cells but stimulated a significant increase (p0.05) in alpha-inhibin production. PGE2 significantly increased progesterone production (p0.001) but inhibited the production of alpha-inhibin (p0.001). After three days of culture, output of alpha-inhibin fell to low levels and no significant effect of hCG or PGE2 was detected. Progesterone also fell with time in culture, but hCG maintained production resulting in a significant increase above control levels (p0.001). The addition of low density lipoproteins (LDL) to the culture medium increased progesterone production (p0.001) while decreasing alpha-inhibin production (p0.01). Immunoneutralization of endogenous alpha-inhibin resulted in a significant decrease in both basal (p0.05) and gonadotropin-stimulated (p0.05) progesterone concentrations. These results provide further evidence for differential control of progesterone and alpha-inhibin production by marmoset luteal cells and show that hCG can selectively stimulate alpha-inhibin production. In addition, alpha-inhibin may have a local paracrine action in the marmoset CL, enhancing both basal and gonadotropin-stimulated progesterone secretion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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