| Autor: |
G B, Afink, M, Nistér, B H, Stassen, P H, Joosten, P J, Rademakers, E, Bongcam-Rudloff, E J, Van Zoelen, S, Mosselman |
| Rok vydání: |
1995 |
| Předmět: |
|
| Zdroj: |
Oncogene. 10(8) |
| ISSN: |
0950-9232 |
| Popis: |
Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. Analysis of 5' promoter deletion mutants revealed that the region from -441 to +118, relative to the transcription initiation site, is sufficient to establish high level promoter activity. In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. This effect is mediated through specific transcription factor binding within the -52/+118 region of the PDGF alpha R gene. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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