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| Autor: | Elsa, Ronzier, Claire, Corratgé-Faillie, Frédéric, Sanchez, Christian, Brière, Tou Cheu, Xiong |
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| Rok vydání: | 2020 |
| Předmět: |
shaker channel
Models Molecular post-translational regulation Patch-Clamp Techniques Arabidopsis thaliana Arabidopsis Proteins Optical Imaging Arabidopsis In Vitro Techniques Plants Genetically Modified calcium signaling Recombinant Proteins Article Plant Leaves CPK Gene Knockout Techniques Xenopus laevis Potassium Channels Voltage-Gated Calcium-Calmodulin-Dependent Protein Kinases Oocytes Potassium Fluorescence Resonance Energy Transfer Animals Female Phosphorylation |
| Zdroj: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | Post-translational regulations of Shaker-like voltage-gated K+ channels were reported to be essential for rapid responses to environmental stresses in plants. In particular, it has been shown that calcium-dependent protein kinases (CPKs) regulate Shaker channels in plants. Here, the focus was on KAT2, a Shaker channel cloned in the model plant Arabidopsis thaliana, where is it expressed namely in the vascular tissues of leaves. After co-expression of KAT2 with AtCPK6 in Xenopus laevis oocytes, voltage-clamp recordings demonstrated that AtCPK6 stimulates the activity of KAT2 in a calcium-dependent manner. A physical interaction between these two proteins has also been shown by Förster resonance energy transfer by fluorescence lifetime imaging (FRET-FLIM). Peptide array assays support that AtCPK6 phosphorylates KAT2 at several positions, also in a calcium-dependent manner. Finally, K+ fluorescence imaging in planta suggests that K+ distribution is impaired in kat2 knock-out mutant leaves. We propose that the AtCPK6/KAT2 couple plays a role in the homeostasis of K+ distribution in leaves. |
| Databáze: | OpenAIRE |
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