Antiviral protection after DNA vaccination is short lived and not enhanced by CpG DNA
| Autor: | S, Oehen, T, Junt, C, López-Macías, T A, Kramps |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cytotoxicity
Immunologic Time Factors Vaccination Epitopes T-Lymphocyte chemical and pharmacologic phenomena Original Articles Lymphocytic Choriomeningitis Peptide Fragments Mice Inbred C57BL Mice Viral Proteins Adjuvants Immunologic Vaccines DNA Animals Lymphocytic choriomeningitis virus CpG Islands Antigens Viral Immunologic Memory Glycoproteins T-Lymphocytes Cytotoxic |
| Zdroj: | Immunology. 99(2) |
| ISSN: | 0019-2805 |
| Popis: | In this study, we investigated the potential of a DNA vaccine expressing the minimal cytotoxic T lymphocyte (CTL) epitope gp33 of the lymphocytic choriomeningitis virus glycoprotein to protect against infection of a non-lymphoid organ and compared this to protection against a systemic infection. Furthermore, since immune stimulatory sequences have been shown to augment CTL responses, we examined the capacity of CpG DNA to enhance CTL memory. The data show that DNA vaccination with a gp33-based gene construct induced short-lived gp33-specific CTL which protected against a systemic infection but not against a peripheral infection. Immune stimulatory sequences were incapable of either prolonging CTL memory or promoting protection against infection of a peripheral organ. |
| Databáze: | OpenAIRE |
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