| Autor: |
Bo, Ma, Jun, Ren, Han-fang, Jiang, Jun, Jia |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences. 40(5) |
| ISSN: |
1671-167X |
| Popis: |
To evaluate the antitumor activities against hepatocellular carcinoma induced by bone marrow mesenchymal stem cells(MSCs) from BALB/c mice pulsed with tumor-derived exosomes.Exosomes derived from malignant ascites of hepatocellular carcinoma were isolated and purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation from BALB/c mice. Bone marrow MSCs from BALB/c mice were obtained using adherent culture method combining with magnetic cell sorting (MACS). IFN-gamma stimulated MSCs were pulsed with tumor-derived exosomes, Then the stimulated MSCs cocultured with H22 hepatocellular carcinoma for 72 hours, the proliferation of H22 hepatocellular carcinoma cells were evaluated.The proliferation of H22 hepatocellular carcinoma cells cocultured with MSCs plused with tumor-derived exosomes, were significantly inhibited (P0.05).When bone marrow MSCs were pulsed with tumor-derived exosomes, MSCs showed enhanced antitumor activities against hepatocellular carcinoma. Therefore, MSCs combined with TEX may offer an effective method to induce antitumor activities. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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