CD40 is required for protective immunity against liver stage Plasmodium infection1
| Autor: | Murray, Sara A, Mohar, Isaac, Miller, Jessica L, Brempelis, Katherine J, Vaughan, Ashley M, Kappe, Stefan HI, Crispe, Ian N |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
animal diseases
Protozoan Proteins Gene Expression chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Lymphocyte Activation Vaccines Attenuated Article Mice Malaria Vaccines Animals CD40 Antigens hemic and immune systems Dendritic Cells Plasmodium yoelii biochemical phenomena metabolism and nutrition Adoptive Transfer Immunity Innate Malaria Mice Inbred C57BL Liver Sporozoites Hepatocytes bacteria Female Gene Deletion Signal Transduction |
| Popis: | The co-stimulatory molecule CD40 enhances immunity through several distinct roles in T cell activation and T cell interaction with other immune cells. In a mouse model of immunity to liver stage Plasmodium infection, CD40 was critical for the full maturation of liver dendritic cells, accumulation of CD8+ T cells in the liver, and protective immunity induced by immunization with the P. yoelii fabb/f- genetically attenuated parasite. Using mixed adoptive transfers of polyclonal wild type (WT) and CD40-deficient (CD40−/−) CD8+ T cells into WT and CD40−/− hosts, we evaluated the contributions to CD8+ T cell immunity of CD40 expressed on host tissues including antigen-presenting cells (APC), compared to CD40 expressed on the CD8+ T cells themselves. Most of the effects of CD40 could be accounted for by expression in the T cells’ environment, including the accumulation of large numbers of CD8+ T cells in the livers of immunized mice. Thus, protective immunity generated during immunization with fabb/f- was largely dependent on effective APC licensing via CD40 signaling. |
| Databáze: | OpenAIRE |
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