| Autor: |
L, Bacáková, V, Mares, M G, Bottone, C, Pellicciari, V, Lisá, V, Svorcík |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
General physiology and biophysics. 18 |
| ISSN: |
0231-5882 |
| Popis: |
The rat vascular (SMCs) and bovine endothelial cells (BECs) were cultured on conventional or fluorine ion-implanted polystyrene (5 x 10(12) and 5 x 10(14) fluorine ions/cm2). The cells grown on the implanted growth supports showed better adherence, higher volume and higher total protein content. The immunocytochemical analysis revealed that SMCs contained more of the cytoskeletal vimentin and the vascular SMC-specific alpha-actin as well as several cell adhesion-mediating molecules (vinculin, talin, alpha(v)-integrin and ICAM-1). In BECs, only the content of vimentin and talin increased, while expression of ICAM-1 was unchanged. The data suggest that cells on the ion implanted polymers could be more viable and that increased expression of some adhesion molecules mediating interactions with the host immune system is cell type-dependent. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
