| Popis: |
To investigate the inhibitive effect of COX-2 inhibitor NS-398 on cancer cell growth of human tongue squamous cell carcinoma.MTT assay was used to study the inhibitive effect of NS-398 on the growth of Tca8113 cells, and to investigate the time-effect relationship and dose-effect relationship between NS-398 and Tca8113 cells. The effect of NS-398 on cell cycle changes of Tca8113 was studied by flow cytometry (FCM). The statistical date were analysed by SAS 8.1 software. Student's t test and repeated-measure ANOVA were used to analyze the date.The Tca8113 cell culture median contained 0, 25, 50, 75, 100, 125, 150, 200 micromol/L NS-398, and individually cultured for 48, 72, 96, 120 hours. The inhibitive effect increased with the time prolonging and the concentration increasing. The effect was most obvious with 150 micromol/L NS-398 cultured for 96 hours (concentration: P0.05, time: P0.001). NS-398 can cause great increase of Tca8113 cells in G0/G1 phase, while cells in G2/M phase decreased, and cells arrested in G0/G1 phase (P0.001).NS-398 can inhibit the growth of Tca8113 cells. The inhibitive effect of NS-398 on Tca8113 cells is dose-dependent and time-dependent, and this effect may be related to the inhibition of cell circle growth. NS-398 may play an important role in the treatment of oral squamous cell carcinoma. |
