Lack of influence of c-Ha-ras expression on the drug sensitivity of human bladder cancer histocultured in three-dimensions
| Autor: | D, Theodorescu, K M, Connors, A, Groce, R M, Hoffman, R S, Kerbel |
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| Rok vydání: | 1993 |
| Předmět: |
Mitomycin
Transplantation Heterologous Drug Resistance Gene Expression Mice Nude Antineoplastic Agents DNA Neoplasm Transfection Mice Genes ras Urinary Bladder Neoplasms Mutagenesis Vincristine Dactinomycin Tumor Cells Cultured Animals Humans Cisplatin Cell Division Neoplasm Transplantation Thymidine |
| Zdroj: | Anticancer research. 13(4) |
| ISSN: | 0250-7005 |
| Popis: | The mechanism of drug resistance in human cancers is complex. In addition to overexpression of a series of multiple-drug-resistance genes, there has been the suggestion that the Ha-ras gene may participate in conferring resistance. In this study, a series of three human-bladder carcinoma cell lines were studied, one parental type, one transfected by wild-type Ha-ras and another transfected by mutant Ha-ras. The ras gene was overexpressed in the latter two cell lines which also were more invasive than the parental when injected as individual cells in the nude-mouse bladder. The results described here have indicated that the ras-gene expression level or mutational status did not affect drug resistance when the tumor lines were histocultured as three-dimensional tissue on collagen-sponge-gels. The drug-response spectrum of the histocultured lines qualitatively reflected a clinical experience although all lines were relatively drug resistant, possibly reflecting their three-dimensional configuration in culture. |
| Databáze: | OpenAIRE |
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