Involvement of transcription factor encoded by the mouse mi locus (MITF) in apoptosis of cultured mast cells induced by removal of interleukin-3
| Autor: | Tsujimura, T., Hashimoto, K., Morii, E., Tunio, G. M., Tsujino, K., Kondo, T., Kanakura, Y., Kitamura, Y. |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Male
Microphthalmia-Associated Transcription Factor integumentary system Apoptosis Cell Count DNA Fragmentation Blotting Northern Immunohistochemistry Gene Expression Regulation Enzymologic Mice Mutant Strains body regions DNA-Binding Proteins Mice Inbred C57BL Mice Animals Female Interleukin-3 Mast Cells Cells Cultured Spleen Research Article DNA Primers Transcription Factors |
| Popis: | Mast cells develop when spleen cells of mice are cultured in the medium containing interleukin (IL)-3. Cultured mast cells (CMCs) show apoptosis when they are incubated in the medium without IL-3. We obtained CMCs from tg/tg mice that did not express the transcription factor encoded by the mi gene (MITF) due to the integration of a transgene at its 5' flanking region. MITF is a member of the basic-helix-loop-helix-leucine zipper (bHLH-Zip) protein family of transcription factors. We investigated the effect of MITF on the apoptosis of CMCs after removal of IL-3. When cDNA encoding normal MITF ((+)-MITF) was introduced into tg/tg CMCs with the retroviral vector, the apoptosis of tg/tg CMCs was significantly accelerated. The mutant mi allele represents a deletion of an arginine at the basic domain of MITF. The apoptosis of tg/tg CMCs was not accelerated by the introduction of cDNA encoding mi-MITF. The overexpression of (+)-MITF was not prerequisite to the acceleration of the apoptosis, as the apoptotic process proceeded faster in +/+ CMCs than in mi/mi CMCs. The Ba/F3 lymphoid cell line is also dependent on IL-3, and Ba/F3 cells show apoptosis after removal of IL-3. The c-myc gene encodes another transcription factor of the bHLH-Zip family, and the overexpression of the c-myc gene accelerated the apoptosis of Ba/F3 cells. However, the overexpression of (+)-MITF did not accelerate the apoptosis of Ba/F3 cells. The (+)-MITF appeared to play some roles for the acceleration of the apoptosis specifically in the mast cell lineage. |
| Databáze: | OpenAIRE |
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