| Autor: |
Bao, Zhang, Xia, Huo, Lin, Peng, Zong-li, Qi, Xi-jin, Xu, Yan, Li, Bo, Qiu, Liang-kai, Zheng |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 27(5) |
| ISSN: |
1673-4254 |
| Popis: |
To construct the prokaryotic plasmid of FUS1 gene for efficient FUS1 expression in E.coli strain Rosetta(DE3)2plys.The full-length FUS1 gene was amplified by PCR from the total RNA of umbilical mesenchymal stem cells and cloned into pET-32a(+) vector followed by identification with PCR and sequencing. The recombinant plasmid pET-32a(+)-FUS1 was transformed into the E.coli strain Rosetta(DE3)2plys and the target protein expression was induced by IPTG.The plasmid pET-32a(+)-FUS1 was obtained successfully as verified by PCR and sequence analysis. High expression of the fused FUS1 protein was achieved after induction by low-concentration IPTG (25 micromol/L) for 3 h, and the recombinant FUS1 protein accounted for 40% of the total bacterial protein of Rosetta(DE3)2plys.The recombinant FUS1 plasmid has been successfully cloned, which allows highly efficient FUS1 expression in Rosetta (DE3)2 plys. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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