| Autor: |
Bo, Han, Zhi-Di, Gao, Hai-Xia, Wang, Zhao-Hui, Wang, Chuan-Bo, Fan, Jun-Ling, Liu, Meng, Tian, Hong-Guo, Zhao |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 27(2) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the expression of miR-155 in patients with diffuse large B-cell lymphoma (DLBCL), and to explore the effect of transfection of miR-155 inhibitor on the biological characteristics of DLBCL cells.A total of 76 patients with DLBCL treated in our hospital were selected from April 2013 to December 2017. In the same time, 40 cases of lymph node reactive hyperplasia (LNRH) were selected as control group. DB cells were cultured and divided into miR-155 inhibitor, negative control and blank groups. The expressions of miR-155 in DLBCL, negative and blank control groups were detected by using real-time PCR, the cell proliferation was detected by MTT assay, the apoptosis was detected by flow cytometry, and the cell migration and invasion were detected by Transwell assay.The relative expression level of miR-155 in tissues of DLBCL patients was significantly higher than that in tissne of controls (1.93±0.16 vs 1.01±0.09) (t=33.991, P=0.000). The expression level of miR-155 increased (P＜0.05) in DLBCL patients with LDH level abnormarity, BCL-2The miR-155 highly expresses in DLBCL tissue, which relates with tumor malignancy and invasion progression. The specific inhibition of miR-155 expression in DB cells can reduce cell proliferation, accelerate cell apoptosis, and inhibit cell migration and invasion.miR-155在弥漫大B细胞淋巴瘤组织中的表达及其对细胞生物学特性的影响.探讨miR-155在弥漫大B细胞淋巴瘤（DLBCL）组织中的表达，以及转染miR-155抑制物对DLBCL细胞生物学特性的影响.选取2013年4月至2017年12月在本院接受治疗的DLBCL患者76例，同时选取因淋巴结反应性增生就诊的40例患者作为对照组。取2组患者组织标本。培养DB细胞并将其分为miR-155抑制物组、阴性对照组和空白组。使用实时荧光定量PCR技术检测DLBCL组和对照组组织及细胞中miR-155的表达；应用MTT法检测细胞的增殖能力，流式细胞术检测细胞凋亡情况；Transwell法检测细胞迁移和侵袭能力.DLBCL患者组织中miR-155相对表达量（1.93±0.16）明显高于对照组（1.01±0.09）（t=33.991，P=0.000）。miR-155表达量在乳酸脱氢酶水平异常、BCL-2miR-155在DLBCL患者组织中呈高表达，且与肿瘤恶性程度和侵袭进展有关，在特异性抑制DB细胞中miR-155的表达可减少细胞增殖，加速细胞凋亡，抑制细胞迁移及侵袭. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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