Cyclooxygenase-2 expression is up-regulated in squamous cell carcinoma of the head and neck
| Autor: | G, Chan, J O, Boyle, E K, Yang, F, Zhang, P G, Sacks, J P, Shah, D, Edelstein, R A, Soslow, A T, Koki, B M, Woerner, J L, Masferrer, A J, Dannenberg |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Transcription
Genetic Reverse Transcriptase Polymerase Chain Reaction Mouth Mucosa Membrane Proteins Gene Expression Regulation Enzymologic Gene Expression Regulation Neoplastic Isoenzymes Cyclooxygenase 2 Head and Neck Neoplasms Prostaglandin-Endoperoxide Synthases Reference Values Carcinoma Squamous Cell Humans RNA Messenger DNA Primers |
| Zdroj: | Cancer research. 59(5) |
| ISSN: | 0008-5472 |
| Popis: | The purpose of this study was to determine whether cyclooxygenase-2 (COX-2) was overexpressed in squamous cell carcinoma of the head and neck (HNSCC). Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in head and neck tissue. Mean levels of COX-2 mRNA were increased by nearly 150-fold in HNSCC (n = 24) compared with normal oral mucosa from healthy volunteers (n = 17). Additionally, there was about a 50-fold increase in amounts of COX-2 mRNA in normal-appearing epithelium adjacent to HNSCC (n = 10) compared with normal oral mucosa from healthy volunteers. Immunoblotting demonstrated that COX-2 protein was present in six of six cases of HNSCC but was undetectable in normal oral mucosa from healthy subjects. Immunohistochemical analysis showed that COX-2 was expressed in both HNSCC and adjacent normal-appearing epithelium. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of HNSCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|