Modeling of the controlled expression of a harmful protein by a three-plasmid harboring system
| Autor: | T V, Unzaga, J C, Díaz-Ricci, J I, Rhee, M R, Hernández, K, Schügerl |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Genetic Transcription Genetic Recombinant Fusion Proteins Staphylococcus Reproducibility of Results Gene Expression Regulation Bacterial Models Biological Sensitivity and Specificity Deoxyribonuclease EcoRI DNA-Binding Proteins Repressor Proteins Kinetics Viral Proteins Models Chemical Species Specificity Protein Biosynthesis Escherichia coli Computer Simulation Viral Regulatory and Accessory Proteins Genetic Engineering Staphylococcal Protein A DNA Damage Plasmids |
| Zdroj: | Biotechnology and bioengineering. 80(5) |
| ISSN: | 0006-3592 |
| Popis: | A genetically structured mathematical model was developed and used to evaluate the influence of molecular parameters involved in the expression of a harmful recombinant protein (SPA::EcoRI). The system consists of the controlled expression of the endonuclease EcoRI cloned in the plasmid pMTC48. The control is exerted by the lambda CI repressor expressed from the plasmid pRK248cIts. The deleterious effect of the activity of the enzyme EcoRI on the host DNA is prevented by the action of the EcoRI methylase that is expressed constitutively from a third plasmid, pEcoR4. The model includes molecular mechanisms involved in the regulation of the expression of these genes and is used to determine cultural conditions that maximize the production of the recombinant protein. |
| Databáze: | OpenAIRE |
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