[Applications of MALDI-TOF-MS in clinical microbiology laboratory]
| Autor: | Etienne, Carbonnelle, Xavier, Nassif |
|---|---|
| Jazyk: | francouzština |
| Rok vydání: | 2011 |
| Předmět: |
Microbiological Techniques
Bacteria Virulence Drug Resistance Fungi Bacterial Infections Bacterial Typing Techniques Specimen Handling Mycoses Species Specificity Virus Diseases Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Virology Viruses Humans Mycological Typing Techniques Biomarkers |
| Zdroj: | Medecine sciences : M/S. 27(10) |
| ISSN: | 0767-0974 |
| Popis: | For twenty years, mass spectrometry (MS) has emerged as a particularly powerful tool for analysis and characterization of proteins in research. It is only recently that this technology, especially MALDI-TOF-MS (Matrix Assisted Laser Desorption Ionization Time-Of-Flight) has entered the field of routine microbiology. This method has proven to be reliable and safe for the identification of bacteria, yeasts, filamentous fungi and dermatophytes. MALDI-TOF-MS is a rapid, precise and cost-effective method for identification, compared to conventional phenotypic techniques or molecular biology. Its ability to analyse whole microorganisms with few sample preparation has greatly reduced the time to identification (1-2 min). Furthermore, this technology can be used to identify bacteria directly from clinical samples as blood culture bottles or urines. Future applications will be developed in order to provide direct information concerning virulence or resistance protein markers. |
| Databáze: | OpenAIRE |
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